![]() Vitro Experiment showed that Methyl Palmitate(MP) can inhibit the proliferation, migration, and invasion of lung cancer cells, and its mechanism of action may be to downregulate the expression of DVL2.įJSF may play a role in inhibiting the occurrence and development of lung cancer by downregulating the expression of DVL2 in A549 cells through its active ingredient Methyl Palmitate. It was negatively correlated with the infiltration of various immune cells in the lung cancer microenvironment. Kaplan-Meier analysis shows that the higher DVL2 expression in lung cancer patients was associated with poorer overall survival and poorer survival in stage Ⅰ patients. Analysis of the data in UCSC to analyze the expression of DVL2 in lung cancer shows that DVL2 was overexpressed in lung adenocarcinoma tissues. Molecular docking shows that the compound Xambioona、quercetin and methyl palmitate in FJSF has a strong binding ability with NTRK1, APC, and DVL2. KEGG pathway enrichment analysis mainly involves PI3K-Akt, TNF, HIF-1, and other pathways. GO enrichment analysis is mainly related to cell migration and movement, lipid metabolism, and protein kinase activity. The results were verified by experiments in vitro.įJSF contained 272 active ingredients and 52 potential targets for lung cancer. Molecular docking was performed by AutoDockTools-1.5.6. xCell method was used to estimate the relationship between DVL2 and immune cell infiltration in lung cancer. Kaplan-Meier Plotter was used to analyze the relationship between DVL2 and the prognosis of lung cancer patients. Cytoscape was used to construct a PPI network and perform topological analysis. GO analysis and KEGG pathway enrichment analysis were performed via the Metascape database. Then drug-disease intersection target genes were obtained through the Venn tool. Disease-related targets of lung cancer were acquired from GeneCards, OMIM, and TTD databases. The "drug-active ingredient-target" network was constructed by Cytoscape. The active components of FJSF were screened by ADME parameters, and the targets were predicted by the Swiss Target Prediction database. To investigate the active components and functional mechanisms of FJSF in treating lung cancer using a network pharmacology approach and molecular docking combined with vitro experiments.īased on the TCMSP and related literature, the chemical components of related herbs in FJSF were collected.
0 Comments
Leave a Reply. |